SUB1 antibody (N-Term)

Catalog No. ABIN2792674

The Rabbit Polyclonal anti-SUB1 antibody is suitable to detect SUB1 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB and IHC.

Quick Overview for SUB1 antibody (N-Term) (ABIN2792674)

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SUB1 Antibody

Binding Specificity: N-Term

Sequence: MPKSKELVSS GSSGSDSDSE VDKKLKRKKQ VAPEKPVKKQ KTGETSRALS

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 91%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against PC4. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PC4

Application Details

Application Notes: WB Suggested Anti-PC4 Antibody Titration: 2.5 µg/mL
Positive Control: Raji cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 127 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SUB1

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Alternative Name: SUB1 / PC4

Background: PC4 is a transcriptional coactivator. General transcription factor IIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity rather than by reducing the repressive activity of PC4 via its phosphorylation
Alias Symbols: P15, PC4, p14
Protein Interaction Partner: HUWE1, SUB1, UBC, rev, CLK4, CDK2, HNF4A, FN1, CSNK2A1, SMURF1, RPL18A, LAMP2, CDC5L, APP, YWHAZ, GTF3C1, ELAVL1, SUMO2, HDGF, Shoc2, Kif1c, POLR2B, POLR2A, CBX5, RCOR1, REST, BANF1, ADAP1, GTF2A1L, EP300, POU2AF1, CSTF2, TBP, TAF1, PCNA, HSF1, PSIP1, SP1
Protein Size: 127

Molecular Weight: 14 kDa

Gene ID: 10923

NCBI Accession: NM_006713, NP_006704

UniProt: P53999