SUB1 antibody (Middle Region)

Catalog No. ABIN2792675

The Rabbit Polyclonal anti-SUB1 antibody (ABIN2792675) specifically detects SUB1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio) and Saccharomyces cerevisiae samples.

Quick Overview for SUB1 antibody (Middle Region) (ABIN2792675)

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SUB1 Antibody

Binding Specificity: Middle Region

Sequence: NMFQIGKMRY VSVRDFKGKV LIDIREYWMD PEGEMKPGRK GISLNPEQWS

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Yeast: 91%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PC4. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PC4

Application Details

Application Notes: WB Suggested Anti-PC4 Antibody Titration: 0.5-1.0 µg/mL
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 127 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SUB1

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Alternative Name: SUB1 / PC4

Background: PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator, possessing the ability to suppress promoter-driven as well as nonspecific transcription via its DNA binding activity. The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH. TFIIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity.
Alias Symbols: P15, PC4, p14
Protein Interaction Partner: HUWE1, SUB1, UBC, rev, CLK4, CDK2, HNF4A, FN1, CSNK2A1, SMURF1, RPL18A, LAMP2, CDC5L, APP, YWHAZ, GTF3C1, ELAVL1, SUMO2, HDGF, Shoc2, Kif1c, POLR2B, POLR2A, CBX5, RCOR1, REST, BANF1, ADAP1, GTF2A1L, EP300, POU2AF1, CSTF2, TBP, TAF1, PCNA, HSF1, PSIP1, SP1
Protein Size: 127

Molecular Weight: 14 kDa

Gene ID: 10923

NCBI Accession: NM_006713, NP_006704

UniProt: P53999