NFATC3 antibody (N-Term)

Catalog No. ABIN2792685

This Rabbit Polyclonal antibody specifically detects NFATC3 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow and Horse.

Quick Overview for NFATC3 antibody (N-Term) (ABIN2792685)

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFATC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NFATC3 Antibody

Binding Specificity: N-Term

Sequence: AVFPFQYCVE TDIPLKTRKT SEDQAAILPG KLELCSDDQG SLSPARETSI

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against NFATC3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NFATC3

Application Details

Application Notes: WB Suggested Anti-NFATC3 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: Hela cell lysate.
Immunohistochemistry with Thymus tissue at an antibody concentration of 5 µg/mL using anti-NFATC3 antibody (ABIN504679).
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1068 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFATC3

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Alternative Name: NFAT4 / NFATC3

Background: NFATC3 is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. NFATC3 plays a role in the regulation of gene expression in T cells and immature thymocytes.The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Four transcript variants encoding distinct isoforms have been identified for this gene.
Alias Symbols: NFAT4, NFATX
Protein Interaction Partner: MAPK9, MAPK8, CDK6, NCOA3, TTF1, CSNK1A1, FOS,
Protein Size: 1068

Molecular Weight: 115 kDa

Gene ID: 4775

NCBI Accession: NM_004555, NP_004546

UniProt: B5B2S0

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Chromatin Binding