Cyclin H antibody (Middle Region)

Catalog No. ABIN2792693

This Rabbit Polyclonal antibody specifically detects Cyclin H in WB. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Rabbit, Horse and Cow.

Quick Overview for Cyclin H antibody (Middle Region) (ABIN2792693)

Target: Cyclin H (CCNH)

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cyclin H Antibody

Binding Specificity: Middle Region

Sequence: KQKLERCHSA ELALNVITKK RKGYEDDDYV SKKSKHEEEE WTDDDLVESL

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 85%, Rabbit: 78%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against CCNH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CCNH

Application Details

Application Notes: WB Suggested Anti-CCNH Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: MCF7 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 323 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Cyclin H

Target: Cyclin H (CCNH)

Alternative Name: Cyclin H

Background: CCNH belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CAK, p34, p37
Protein Interaction Partner: SFN, GOLGA2, SSX2IP, CCDC170, CCDC33, DUSP12, SSSCA1, NDC80, CALCOCO2, PPFIA1, PSMA1, KLC3, RPA3, RPA2, RPA1, NEDD4, CDK7, CDK8, CDK2, CCNC, MNAT1, MBP, CTD, CDK20, CTBP2, CSNK2B, TRIM8, GANAB, RARB, POLR2A, GTF2H4, ERCC2, tat, POLR2B, CDK9, BRCA1, GTF2H1
Protein Size: 323

Molecular Weight: 38 kDa

Gene ID: 902

NCBI Accession: NM_001239, NP_001230

UniProt: P51946

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase