Actin antibody (Center)

Catalog No. ABIN2798817

This anti-Actin antibody is a Rabbit Polyclonal antibody detecting Actin in WB and IHC. Suitable for Human.

Quick Overview for Actin antibody (Center) (ABIN2798817)

Target: Actin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Actin Antibody

Binding Specificity: AA 189-218, Center

Purpose: Rabbit Anti-Human Actin (ACTB/ACTC) (Center) Antibody

Immunogen: This Actin (ACTB/ACTC) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the Central region of human Actin (ACTB/ACTC).

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50Western blot analysis of ACTB/ACTC Antibody (Center) in HL60 cell line lysates (35 μg/lane). Target protein (arrow) was detected using the purified Pab.Western blot analysis of ACTB/ACTC (arrow) using rabbit polyclonal ACTB/ACTC Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for Actin

Target: Actin

Background:

Target Description: Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

Gene Symbol: ACTC1

Molecular Weight: 42019 Da

Gene ID: 70

UniProt: P68032