GCAP1 antibody (C-Term)

Catalog No. ABIN2798879

Product Details anti-GCAP1 Antibody

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Binding Specificity: AA 160-191, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Rabbit Anti-Human GCAP1 (C-term) Antibody

Immunogen: This GCAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-191 amino acids from the C-terminal region of human GCAP1.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100The anti-GCAP1 C-term Pab is used in Western blot to detect GCAP1 in Y79 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated. BC = breast carcinoma, HC = hepatocarcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for GCAP1

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Alternative Name: GCAP1 (GUCA1A Products)

Synonyms: C6orf131 antibody, COD3 antibody, CORD14 antibody, GCAP antibody, GCAP1 antibody, GUCA antibody, GUCA1 antibody, dJ139D8.6 antibody, MGC84170 antibody, GUCA1A antibody, guca1a antibody, MGC146317 antibody, GC-A antibody, Gcap1 antibody, Guca1 antibody, mGCAP1 antibody, gcap1 antibody, guanylate cyclase activator 1A antibody, guanylate cyclase activator 1A L homeolog antibody, guanylate cyclase activator 1A (retina) antibody, guanylate cyclase activator 1a (retina) antibody, GUCA1A antibody, Guca1a antibody, guca1a.L antibody, guca1a antibody

Background:

Target Description: Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90 % similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.

Gene Symbol: GUCA1A

Molecular Weight: 22920 Da

Gene ID: 2978

UniProt: P43080

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction