SIGLEC11 antibody (C-Term)

Catalog No. ABIN2799004

Product Details anti-SIGLEC11 Antibody

Target: SIGLEC11 (Sialic Acid Binding Ig-Like Lectin 11 (SIGLEC11))

Binding Specificity: AA 631-662, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC11 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Purpose: Rabbit Anti-Human SIGLEC11 (C-term) Antibody

Immunogen: This SIGLEC11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 631-662 amino acids from the C-terminal region of human SIGLEC11.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50SIGLEC11 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.310 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for SIGLEC11

Target: SIGLEC11 (Sialic Acid Binding Ig-Like Lectin 11 (SIGLEC11))

Alternative Name: SIGLEC11 (SIGLEC11 Products)

Synonyms: sialic acid binding Ig like lectin 11 antibody, SIGLEC11 antibody

Background:

Target Description: SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have one or more cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIM). SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and four C2-set Ig-like domains, five potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8. Expression was also detected on monocytes and a CD16-positive/CD56-negative natural killer-like lymphocyte population. After sialidase treatment, which is necessary for unmasking the sialic acid-binding site on SIGLECs interacting with cell surface sialic acids, cells expressing SIGLEC10 bound to red blood cells. Immunoprecipitation analysis indicated expression of a 100- to 120-kD monomeric protein, higher than the predicted molecular mass, suggesting that SIGLEC10 is glycosylated.

Gene Symbol: SIGLEC11

Molecular Weight: 75795 Da

Gene ID: 114132

UniProt: Q96RL6