Tested Western blot Application Notes Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.Tim17 antibody [C1C3] detects Tim17 protein at mitochondria by immunofluorescent analysis.Sample: A549 cells were fixed in ice-cold MeOH for 5 min.Green: Tim17 protein stained by Tim17 antibody [C1C3] (107-10750) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 ?m.Sample (30 μg of whole cell lysate) A: Hela 12 % SDS PAGE 107-10750 diluted at 1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
-20°C
Target
TIMM17A
(Translocase of Inner Mitochondrial Membrane 17 Homolog A (TIMM17A))