SQSTM1 antibody (C-Term)

Catalog No. ABIN2800638

This anti-SQSTM1 antibody is a Rabbit Polyclonal antibody detecting SQSTM1 in WB, IHC and IF. Suitable for Human.

Quick Overview for SQSTM1 antibody (C-Term) (ABIN2800638)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SQSTM1 Antibody

Binding Specificity: AA 317-346, C-Term

Purpose: Rabbit Anti-Human SQSTM1 (p62) (C-term) Antibody

Immunogen: This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human SQSTM1 (p62).

Isotype: Ig Fraction

Application Details

Application Notes: Immunofluorescence, Western Blot, Immunohistochemistry
Recommended Dilutions
IF: 1:50-100, WB: 1:1000, IHC: 1:50-100Fluorescent image of U251 cells stained with SQSTM1 (p62) (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4 % PFA (20 min), permeabilized with Triton X-100 (0.2 %, 30 min). Cells were then incubated with 102-15010 SQSTM1 (p62) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/mL, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000161011).Western blot analysis of SQSTM1 Antibody (C331) Pab on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background:

Gene Symbol: SQSTM1

Molecular Weight: 47687 Da

Gene ID: 8878

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy