Fluorescein antibody

Catalog No. ABIN2801850

Product Details

Target: Fluorescein

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA

Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Fluorescein conjugated Rabbit IgG and Fluorescein conjugated KLH.

Characteristics: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Sterility: Sterile filtered

Immunogen: Fluorescein conjugated Bovine Serum Albumin (BSA)
Immunogen Type: NativeProtein

Application Details

Application Notes: This product has been assayed against 1.0 ug of Fluorescein conjugated Rabbit IgG in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] Donkey) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:100,000 to 1:500,000 of the reconstitution concentration is suggested for this product. 

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 90 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References

Kotani, Yamaguchi, Ohnishi, Kuwahara, Nakano, Nakano, Ida, Murakoshi, Honke: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).

Miyagawa-Yamaguchi, Kotani, Honke: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).

Target Details

Target: Fluorescein

Target Type: Chemical

Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms: FITC