ALAS2 antibody (AbBy Fluor® 594)

Catalog No. ABIN2809602

This Rabbit Polyclonal antibody specifically detects ALAS2 in WB and IF (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ALAS2 antibody (AbBy Fluor® 594) (ABIN2809602)

Target: ALAS2 (Aminolevulinate, delta-, Synthase 2 (ALAS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAS2 antibody is conjugated to AbBy Fluor® 594

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ALAS2 Antibody (AbBy Fluor® 594)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ALAS2/ALAS-E

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ALAS2

Target: ALAS2 (Aminolevulinate, delta-, Synthase 2 (ALAS2))

Alternative Name: ALAS-E

Background:

Synonyms: 5-aminolevulinate synthase, erythroid-specic, mitochondrial, 5-aminolevulinic acid synthase, ALAS E, ALASE, ANH1, Delta aminolevulinate synthase, XLSA, 5 aminolevulinic acid synthase 2, 5-aminolevulinate synthase 2, 5-aminolevulinate synthase, 5-aminolevulinate synthase 2, Alas 2, ALAS, ALAS E, ALAS, erythroid, ALASE, Aminolevulinate, delta-, synthase 2, Aminolevulinic acid synthase 2, erythroid, ANH1, ASB, Delta ALA synthase 2, Delta ALA synthetase, Delta aminolevulinate synthase 2, Delta aminolevulinate synthase, Erythroid specic ALAS, FLJ93603, XLDPP, XLSA.

Background: 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.

Gene ID: 212

Pathways: Transition Metal Ion Homeostasis