RYK antibody (AA 160-190)

Catalog No. ABIN2841818

This Rabbit Polyclonal antibody specifically detects RYK in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 4+ publications.

Quick Overview for RYK antibody (AA 160-190) (ABIN2841818)

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RYK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1491

Product Details anti-RYK Antibody

Binding Specificity: AA 160-190

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 amino acids from human RYK.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-RYK antibody (ABIN2841818)

Povinelli, Nemeth: "Wnt5a regulates hematopoietic stem cell proliferation and repopulation through the Ryk receptor." in: Stem cells (Dayton, Ohio), Vol. 32, Issue 1, pp. 105-15, (2014) (PubMed).

González, Fernández-Martos, Arenas, Rodríguez: "The Ryk receptor is expressed in glial and fibronectin-expressing cells after spinal cord injury." in: Journal of neurotrauma, Vol. 30, Issue 10, pp. 806-17, (2013) (PubMed).

Santiago, Oguma, Brown, Laurence: "Noncanonical Wnt signaling promotes osteoclast differentiation and is facilitated by the human immunodeficiency virus protease inhibitor ritonavir." in: Biochemical and biophysical research communications, Vol. 417, Issue 1, pp. 223-30, (2012) (PubMed).

Miyashita, Koda, Kitajo, Yamazaki, Takahashi, Kikuchi, Yamashita: "Wnt-Ryk signaling mediates axon growth inhibition and limits functional recovery after spinal cord injury." in: Journal of neurotrauma, Vol. 26, Issue 7, pp. 955-64, (2009) (PubMed).

Target Details for RYK

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Alternative Name: RYK

Background: RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. A nine nucleotide insertion in some transcripts results in the SLG variant. It is not established whether this is a product of alternative splicing or a second gene, since evidence for a second gene or pseudogene on chromosome 17 exists.

Molecular Weight: 67815

Gene ID: 6259

NCBI Accession: NP_001005861, NP_002949

UniProt: P34925

Pathways: RTK Signaling, WNT Signaling, Regulation of Cell Size