CD24 antibody (N-Term)

Catalog No. ABIN652728

The Rabbit Polyclonal anti-CD24 antibody (Clone RB23639) (ABIN652728) specifically detects CD24 in FACS, WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CD24 antibody (N-Term) (ABIN652728)

Target: CD24 (CD24 Molecule (CD24))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD24 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB23639

Product Details anti-CD24 Antibody

Binding Specificity: AA 1-30, N-Term

Predicted Reactivity: Mouse (Murine)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CD24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human CD24.

Isotype: Ig Fraction

Application Details

Application Notes: Suggested dilutions:
WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

Target Details for CD24

Target: CD24 (CD24 Molecule (CD24))

Alternative Name: CD24

Background: Function:
Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.

Cellular Location:
Cell membrane, Lipid-anchor, GPI-anchor.

Tissue Location:
B-cells. Expressed in a number of B-cell lines including P32/ISH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells

Synonyms: CD_antigen=CD24, Flags: Precursor, Small cell lung carcinoma cluster 4 antigen, Signal transducer CD24, CD24, CD24A

Molecular Weight: 8097 Da

Gene ID: 100133941

NCBI Accession: NP_037362

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation