BrdU antibody

Catalog No. ABIN285018

Cited in 10+ publications. The Sheep Polyclonal anti-BrdU antibody (ABIN285018) specifically detects BrdU in IHC, IP and IC.

Quick Overview for BrdU antibody (ABIN285018)

Target: BrdU (Bromodeoxyuridine (BrdU))

Host: Sheep

Clonality: Polyclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunochromatography (IC)

Product Details anti-BrdU Antibody

Cross-Reactivity (Details): Not species specific. Nucleotide is present in all species. Very low cross reactivity to 5-methyl cytosine.

Purification: purified

Immunogen: BRDU antibody was raised in sheep using BrdU coupled to KLH as the immunogen.

Application Details

Application Notes: IC: 10 µg/mL, IHC: 10 µg/mL, IP: 25-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Protein G purified and supplied in PBS buffer, with no preservative.

Preservative: Without preservative

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20 °C for short term storage, -70 °C for long-term storage.

References for anti-BrdU antibody (ABIN285018)

Douvaras, Dorà, Mort, Lodge, Hill, West: "Abnormal corneal epithelial maintenance in mice heterozygous for the micropinna microphthalmia mutation Mp." in: Experimental eye research, Vol. 149, pp. 26-39, (2017) (PubMed).

Simitsidellis, Gibson, Cousins, Esnal-Zufiaurre, Saunders: "A Role for Androgens in Epithelial Proliferation and Formation of Glands in the Mouse Uterus." in: Endocrinology, Vol. 157, Issue 5, pp. 2116-28, (2017) (PubMed).

Vitte, Gao, Coppola, Judkins, Giovannini: "Timing of Smarcb1 and Nf2 inactivation determines schwannoma versus rhabdoid tumor development." in: Nature communications, Vol. 8, Issue 1, pp. 300, (2017) (PubMed).

Chang, Morrison, Nilsson, Kenyon, West, Morley: "Cell proliferation, movement and differentiation during maintenance of the adult mouse adrenal cortex." in: PLoS ONE, Vol. 8, Issue 12, pp. e81865, (2014) (PubMed).

Sountoulidis, Stavropoulos, Giaglis, Apostolou, Monteiro, Chuva de Sousa Lopes, Chen, Stripp, Mummery, Andreakos, Sideras: "Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair." in: PLoS ONE, Vol. 7, Issue 8, pp. e41460, (2012) (PubMed).

Schultz, Banisadr, Lastra, McGuire, Kessler, Miller, McGarry: "Geminin-deficient neural stem cells exhibit normal cell division and normal neurogenesis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17736, (2011) (PubMed).

Ashley, Grace, Vanpoucke, Thomson: "Identification of EphrinB1 expression in prostatic mesenchyme and a role for EphB-EphrinB signalling in prostate development." in: Differentiation; research in biological diversity, Vol. 80, Issue 2-3, pp. 89-98, (2010) (PubMed).

Jabès, Lavenex, Amaral, Lavenex: "Quantitative analysis of postnatal neurogenesis and neuron number in the macaque monkey dentate gyrus." in: The European journal of neuroscience, Vol. 31, Issue 2, pp. 273-85, (2010) (PubMed).

Tavazoie, Van der Veken, Silva-Vargas, Louissaint, Colonna, Zaidi, Garcia-Verdugo, Doetsch: "A specialized vascular niche for adult neural stem cells." in: Cell stem cell, Vol. 3, Issue 3, pp. 279-88, (2008) (PubMed).

Freestone, Marker, Grace, Tomlinson, Cunha, Harnden, Thomson: "Sonic hedgehog regulates prostatic growth and epithelial differentiation." in: Developmental biology, Vol. 264, Issue 2, pp. 352-62, (2003) (PubMed).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BRDU

Target Type: Chemical

Background: Bromodeoxyuridine is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical.