POLE3 antibody

Catalog No. ABIN363202

This Mouse Monoclonal antibody specifically detects POLE3 in WB, IF, IP and ICC. It exhibits reactivity toward Human, Mouse and Monkey.

Quick Overview for POLE3 antibody (ABIN363202)

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLE3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 3C5-1

Product Details anti-POLE3 Antibody

Specificity: Reacts with DNA polymerase epsilon from human mouse, monkey, mink and hamster

Cross-Reactivity (Details): Does not recognize DNA pol alpha, beta or delta. Does not reacts with Drosophilia or baculovirus host cell pol epsilon, and weakly, if at all, on S. cerivisae pol epsilon.

Purification: Purified (protein G)

Immunogen: Human DNA Polymerase epsilon purified from HeLa cells

Isotype: IgG2a

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC/IF1:100, IP1:10-1:500, WB5 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 16 mg/mL

Buffer: PBS

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for POLE3

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Alternative Name: DNA Polymerase epsilon Subunit 3

Background: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.

Gene ID: 54107

UniProt: Q9NRF9

Pathways: DNA Damage Repair