FAP antibody

Catalog No. ABIN2854402

This Rabbit Polyclonal antibody specifically detects FAP in WB. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for FAP antibody (ABIN2854402)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FAP Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to FAP (fibroblast activation protein, alpha)
FAP antibody [N1N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human FAP. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2 , Molt-4

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.58 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-FAP antibody (ABIN2854402)

Wolczyk, Zaremba-Czogalla, Hryniewicz-Jankowska, Tabola, Grabowski, Sikorski, Augoff: "TNF-α promotes breast cancer cell migration and enhances the concentration of membrane-associated proteases in lipid rafts." in: Cellular oncology (Dordrecht), Vol. 39, Issue 4, pp. 353-63, (2017) (PubMed).

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: fibroblast activation protein alpha

Background: The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.

Cellular Localization: Cell membrane, Single-pass type II membrane protein , Cell projection , lamellipodium membrane, Single-pass type II membrane protein , invadopodium membrane, Single-pass type II membrane protein

Molecular Weight: 88 kDa

Gene ID: 2191

UniProt: Q12884

Pathways: Tube Formation