RAP1A antibody (Center)

Catalog No. ABIN2854404

Product Details anti-RAP1A Antibody

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAP1A antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Mouse (100 %), Rat (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit polyclonal antibody to RAP1A (RAP1A, member of RAS oncogene family)
RAP1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RAP1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.53 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-RAP1A antibody (ABIN2854404)

Chinigò, Grolez, Audero, Bokhobza, Bernardini, Cicero, Toillon, Bailleul, Visentin, Ruffinatti, Brysbaert, Lensink, De Ruyck, Cantelmo, Fiorio Pla, Gkika: "TRPM8-Rap1A Interaction Sites as Critical Determinants for Adhesion and Migration of Prostate and Other Epithelial Cancer Cells." in: Cancers, Vol. 14, Issue 9, (2022) (PubMed).

Target Details for RAP1A

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Alternative Name: RAP1 (RAP1A Products)

Background: The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50 % amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

Cellular Localization: Cell membrane, Lipid-anchor

Molecular Weight: 21 kDa

Gene ID: 5906

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor