APEH antibody (Center)

Catalog No. ABIN2854516

This Rabbit Polyclonal antibody specifically detects APEH in WB. It exhibits reactivity toward Human.

Quick Overview for APEH antibody (Center) (ABIN2854516)

Target: APEH (N-Acylaminoacyl-Peptide Hydrolase (APEH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APEH Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Pig (Porcine), Rat (Rattus)

Cross-Reactivity (Details): Mouse (88 %), Pig (89 %), Rat (89 %)

Characteristics: Rabbit polyclonal antibody to APEH (N-acylaminoacyl-peptide hydrolase)
APEH antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human APEH. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.34 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for APEH

Target: APEH (N-Acylaminoacyl-Peptide Hydrolase (APEH))

Alternative Name: APEH

Background: This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.

Cellular Localization: Cytoplasm

Molecular Weight: 81 kDa

Gene ID: 327