PLAU antibody

Catalog No. ABIN2854759

This Rabbit Polyclonal antibody specifically detects PLAU in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PLAU antibody (ABIN2854759)

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLAU antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLAU Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to Urokinase (plasminogen activator, urokinase)
Urokinase antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Urokinase. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299 , HCT116 , PC3 conditioned medium

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PLAU

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Alternative Name: plasminogen activator, urokinase

Background: This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Secreted

Molecular Weight: 49 kDa

Gene ID: 5328

UniProt: P00749

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration