PARP1 antibody

Catalog No. ABIN2854798

This Rabbit Polyclonal antibody specifically detects PARP1 in WB, IP, IF, ICC, IHC (p) and ChIP. It exhibits reactivity toward Human and has been mentioned in 7+ publications.

Quick Overview for PARP1 antibody (ABIN2854798)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Grade: KO Validated

Product Details anti-PARP1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to PARP (poly (ADP-ribose) polymerase 1)
PARP antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HCT116 (30uM cisplatin treatment for 24hr) , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2

Validation: Comparison, KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PARP1 antibody (ABIN2854798)

Takino, Sato, Nagamine, Hori: "The inhibition of Bax activation-induced apoptosis by RasGRP2 via R-Ras-PI3K-Akt signaling pathway in the endothelial cells." in: Scientific reports, Vol. 9, Issue 1, pp. 16717, (2019) (PubMed).

Bera, Chiu, Huang, Liang, Lee, Shih: "Genetic and Epigenetic Perturbations by DNMT3A-R882 Mutants Impaired Apoptosis through Augmentation of PRDX2 in Myeloid Leukemia Cells." in: Neoplasia (New York, N.Y.), Vol. 20, Issue 11, pp. 1106-1120, (2019) (PubMed).

Dominguez, Maddelein, Pucelle, Nicaise, Maurage, Duyckaerts, Cuvillier, Delisle: "Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain." in: Acta neuropathologica communications, Vol. 6, Issue 1, pp. 25, (2019) (PubMed).

Li, Chen, Chen, Yen, Fang, Tsai, Chang, Shen, Huang, Chuu, Chang, Hsiung, Jiang: "Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma." in: Scientific reports, Vol. 7, Issue 1, pp. 11893, (2019) (PubMed).

Chakraborty, Tapryal, Venkova, Horikoshi, Pandita, Sarker, Sarkar, Pandita, Hazra: "Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes." in: Nature communications, Vol. 7, pp. 13049, (2018) (PubMed).

Liang, Shi, Yang, Qiu, Lin, Qi, Li, Zhao, Liu: "Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells." in: British journal of cancer, Vol. 116, Issue 7, pp. 903-911, (2017) (PubMed).

Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." in: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: poly(ADP-ribose) polymerase 1

Background: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Cellular Localization: Nucleus

Molecular Weight: 113 kDa

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location