MTDH antibody

Catalog No. ABIN2854801

This Rabbit Polyclonal antibody specifically detects MTDH in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for MTDH antibody (ABIN2854801)

Target: MTDH (Metadherin (MTDH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-MTDH Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit polyclonal antibody to LYRIC (metadherin)
LYRIC antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human LYRIC . The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.69 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-MTDH antibody (ABIN2854801)

Lai, Chuang, Liu, Ho, Lin, Anderson, Yeh: "Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection." in: Scientific reports, Vol. 7, Issue 1, pp. 6975, (2019) (PubMed).

Target Details for MTDH

Target: MTDH (Metadherin (MTDH))

Alternative Name: metadherin

Background: Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

Cellular Localization: Endoplasmic reticulum membrane, Single-pass membrane protein , Nucleus membrane, Single-pass membrane protein (By similarity) , Cell junction , tight junction (By similarity) , Nucleus , nucleolus (By similarity) , Cytoplasm , perinuclear region

Molecular Weight: 64 kDa

Gene ID: 92140

UniProt: Q86UE4

Pathways: Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization