MUC2 antibody (C-Term)

Catalog No. ABIN2854828

This Rabbit Polyclonal antibody specifically detects MUC2 in IHC, WB, IF, ICC, IHC (p), IHC (fro) and FISH. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for MUC2 antibody (C-Term) (ABIN2854828)

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Fluorescence in situ hybridization (FISH)

Product Details anti-MUC2 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to MUC2 (mucin 2, oligomeric mucus/gel-forming)
MUC2 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MUC2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-MUC2 antibody (ABIN2854828)

Toubai, Fujiwara, Rossi, Riwes, Tamaki, Zajac, Liu, Mathew, Byun, Oravecz-Wilson, Matsuda, Sun, Peltier, Wu, Chen, Seregin, Henig, Kim, Brabbs, Pennathur, Chen, Reddy: "Host NLRP6 exacerbates graft-versus-host disease independent of gut microbial composition." in: Nature microbiology, Vol. 4, Issue 5, pp. 800-812, (2019) (PubMed).

Target Details for MUC2

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Alternative Name: mucin 2, oligomeric mucus/gel-forming

Background: This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80 % is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Cellular Localization: Secreted

Molecular Weight: 540 kDa

Gene ID: 4583

UniProt: Q02817