MUC2 antibody

Catalog No. ABIN6940118

This Mouse Monoclonal antibody specifically detects MUC2 in IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for MUC2 antibody (ABIN6940118)

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: MLP-842

Product Details anti-MUC2 Antibody

Purpose: MUC2 (Mucin 2) Antibody

Specificity: Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium.MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

No Cross-Reactivity: Rat (Rattus)

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment covering tandem repeats of human MUC2 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: LS174T cells. Human small intestine (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris-HCl buffer, pH 10.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MUC2

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Alternative Name: MUC2

Background:

Mucin-2, Intestinal mucin-2, Mucin like protein, (MLP), SMUC

LS174T cells. Human small intestine (IHC).

Molecular Weight: 520kDa

Gene ID: 4583, 315

UniProt: Q02817