Cystatin S (CST4) (N-Term) antibody

Details for Product No. ABIN2854838
Antigen
Epitope
N-Term
16
6
3
Reactivity
Human
81
10
2
Host
Rabbit
51
30
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
43
14
14
13
8
7
7
6
4
3
2
2
1
1
Options
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cystatin S. The exact sequence is proprietary.
Isotype IgG
Characteristics Rabbit Polyclonal antibody to Cystatin S (cystatin S)
Cystatin S antibody [N1N3]
Purification Purified by antigen-affinity chromatography.
Antigen
Alternative Name Cystatin S (CST4 Antibody Abstract)
Background The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function.

Cellular Localization: Secreted
Molecular Weight 16 kDa
Gene ID 1472
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Cystatin S (CST4) (N-Term) antibody (ABIN2854838) WB Image Sample (30 ug of whole cell lysate) A: Hep G2 , 15% SDS PAGE antibody dilute...
Image no. 2 for anti-Cystatin S (CST4) (N-Term) antibody (ABIN2854838) ICC/IF Image Immunofluorescence analysis of methanol-fixed A431, using Cystatin S, an...
Image no. 3 for anti-Cystatin S (CST4) (N-Term) antibody (ABIN2854838) IHC-P Image Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using ...