MMP12 antibody

Catalog No. ABIN2854843

This Rabbit Polyclonal antibody specifically detects MMP12 in WB, ICC, IHC (p) and IF. It exhibits reactivity toward Human.

Quick Overview for MMP12 antibody (ABIN2854843)

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP12 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-MMP12 Antibody

Cross-Reactivity: Human, Rat

Characteristics: Rabbit Polyclonal antibody to MMP12 (matrix metallopeptidase 12 (macrophage elastase))
MMP12 antibody [N3C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP12. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MMP12

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Alternative Name: matrix metallopeptidase 12

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Cellular Localization: Secreted , extracellular space , extracellular matrix

Molecular Weight: 54 kDa

Gene ID: 4321

UniProt: P39900