NYS48/HAUS8 antibody

Catalog No. ABIN2854852

Product Details anti-NYS48/HAUS8 Antibody

Target: NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NYS48/HAUS8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to HICE1 (HAUS augmin-like complex, subunit 8)
HICE1 antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein corresponding to human HICE1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:50-1:500. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U2OS

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.17 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NYS48/HAUS8

Target: NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))

Alternative Name: HAUS augmin like complex subunit 8 (HAUS8 Products)

Synonyms: 2410004L22Rik antibody, Hice1 antibody, NY-SAR-48 antibody, hice1 antibody, DGT4 antibody, HICE1 antibody, Ny-sar-48 antibody, RGD1311565 antibody, 4HAUS augmin-like complex, subunit 8 antibody, HAUS augmin like complex subunit 8 L homeolog antibody, HAUS augmin like complex subunit 8 antibody, HAUS augmin-like complex, subunit 8 antibody, Haus8 antibody, haus8.L antibody, HAUS8 antibody

Background: HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220], Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM]

Cellular Localization: Cytoplasm , Centrosome , Spindle

Molecular Weight: 45 kDa

Gene ID: 93323

UniProt: Q9BT25