SYK antibody (Internal Region)

Catalog No. ABIN2854855

This Rabbit Polyclonal antibody specifically detects SYK in WB, IF, IP, IHC (p) and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SYK antibody (Internal Region) (ABIN2854855)

Target: SYK (Spleen tyrosine Kinase (SYK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-SYK Antibody

Binding Specificity: Internal Region

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Bovine (80 %)

Characteristics: Rabbit Polyclonal antibody to SYK (spleen tyrosine kinase)
SYK antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SYK. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: A431 , Raji , BMDM , mouse spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.85 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: SYK

Background: This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 72 kDa

Gene ID: 6850

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling