RAC1 antibody (Center)

Catalog No. ABIN2854858

This Rabbit Polyclonal antibody specifically detects RAC1 in WB and IHC (p). It exhibits reactivity toward Human and Mouse and has been mentioned in 2+ publications.

Quick Overview for RAC1 antibody (Center) (ABIN2854858)

Target: RAC1

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAC1 Antibody

Binding Specificity: Center

Cross-Reactivity: Chicken, Dog (Canine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Chicken (90 %), Dog (90 %), Pig (90 %), Rat (90 %), Xenopus laevis (90 %), Bovine (90 %), Xenopus tropicalis (90 %)

Characteristics: Rabbit Polyclonal antibody to RAC1 (ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1))
RAC1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RAC1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , Molt-4 , Raji , mouse liver

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-RAC1 antibody (ABIN2854858)

Wang, Hsu, Ko, Chiu, Kan, Lai, Wang: "Astrocytic CCAAT/Enhancer-Binding Protein Delta Contributes to Glial Scar Formation and Impairs Functional Recovery After Spinal Cord Injury." in: Molecular neurobiology, Vol. 53, Issue 9, pp. 5912-5927, (2018) (PubMed).

Katz, Sims, Sproul, Caldwell, Dixon, Meehan, Harrison: "Targeting of Rac GTPases blocks the spread of intact human breast cancer." in: Oncotarget, Vol. 3, Issue 6, pp. 608-19, (2012) (PubMed).

Target Details for RAC1

Target: RAC1

Background: The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cell membrane, Lipid-anchor, Cytoplasmic side (By similarity) , Melanosome

Molecular Weight: 21 kDa

Gene ID: 5879

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling