Cyclin B1 antibody

Catalog No. ABIN2854892

The Rabbit Polyclonal anti-Cyclin B1 antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect Cyclin B1 in samples from Human. There are 3+ publications available.

Quick Overview for Cyclin B1 antibody (ABIN2854892)

Target: Cyclin B1 (CCNB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Cyclin B1 Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to Cyclin B1 (cyclin B1)
Cyclin B1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Cyclin B1 antibody (ABIN2854892)

Luo, Liao, Liao, Chen, Huang, Fan, Liao: "The role of kinesin KIF18A in the invasion and metastasis of hepatocellular carcinoma." in: World journal of surgical oncology, Vol. 16, Issue 1, pp. 36, (2018) (PubMed).

Kuo, Li, Stark, Shih, Ann: "RNF4 regulates DNA double-strand break repair in a cell cycle-dependent manner." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 6, pp. 787-98, (2016) (PubMed).

Li, Wu, Lee, Li, Hsieh, Ho, Ho, Chen, Wang, Yen, Yang, Chen: "Hinokitiol induces DNA damage and autophagy followed by cell cycle arrest and senescence in gefitinib-resistant lung adenocarcinoma cells." in: PLoS ONE, Vol. 9, Issue 8, pp. e104203, (2015) (PubMed).

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: cyclin B1

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Cellular Localization: Cytoplasm , Nucleus , Centrosome

Molecular Weight: 48 kDa

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase