Angiopoietin 2 antibody

Catalog No. ABIN2854896

This anti-Angiopoietin 2 antibody is a Rabbit Polyclonal antibody detecting Angiopoietin 2 in WB and IHC (p). Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Angiopoietin 2 antibody (ABIN2854896)

Target: Angiopoietin 2 (ANGPT2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiopoietin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Angiopoietin 2 Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to Angiopoietin 2 (angiopoietin-2)
Angiopoietin 2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Angiopoietin 2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.04 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Angiopoietin 2 antibody (ABIN2854896)

Gu, Zhang, Han, Gao, Cui, Sun, Niu, You, Huang, Chang, Wang, Yeh: "Targeting the ERβ/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC." in: Cell death & disease, Vol. 11, Issue 5, pp. 367, (2020) (PubMed).

Fischer, Seifen, Baer, Jung, Mertens, Scheller, Zacharowski, Hofmann, Maier, Urbschat: "The Fibrin Cleavage Product Bβ15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury." in: Frontiers in pharmacology, Vol. 9, pp. 369, (2018) (PubMed).

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: angiopoietin 2

Background: The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.

Cellular Localization: Secreted

Molecular Weight: 57 kDa

Gene ID: 285

UniProt: O15123

Pathways: RTK Signaling