SEC13 antibody (Center)

Catalog No. ABIN2854912

This Rabbit Polyclonal antibody specifically detects SEC13 in WB, IP, IF, IHC (p) and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for SEC13 antibody (Center) (ABIN2854912)

Target: SEC13 (SEC13 Homolog (SEC13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEC13 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-SEC13 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Mouse (97 %), Rat (97 %), Xenopus laevis (88 %), Zebrafish (87 %), Bovine (96 %), Xenopus tropicalis (89 %)

Characteristics: Rabbit Polyclonal antibody to SEC13L1 (SEC13 homolog (S. cerevisiae))
SEC13L1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SEC13L1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-SEC13 antibody (ABIN2854912)

Wolfson, Chantranupong, Wyant, Gu, Orozco, Shen, Condon, Petri, Kedir, Scaria, Abu-Remaileh, Frankel, Sabatini: "KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1." in: Nature, Vol. 543, Issue 7645, pp. 438-442, (2017) (PubMed).

Target Details for SEC13

Target: SEC13 (SEC13 Homolog (SEC13))

Alternative Name: SEC13L1

Background: The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found.

Molecular Weight: 36 kDa

Gene ID: 6396