ube3a antibody

Catalog No. ABIN2854930

This Rabbit Polyclonal antibody specifically detects ube3a in IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for ube3a antibody (ABIN2854930)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ube3a antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-ube3a Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to UBE3A (ubiquitin protein ligase E3A)
UBE3A antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human UBE3A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.26 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: ubiquitin protein ligase E3A

Background: This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Cellular Localization: Nucleus

Molecular Weight: 100 kDa

Gene ID: 7337

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway