MLH1 antibody

Catalog No. ABIN2854956

This anti-MLH1 antibody is a Rabbit Polyclonal antibody detecting MLH1 in WB, IF and ICC. Suitable for Human.

Quick Overview for MLH1 antibody (ABIN2854956)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MLH1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to MLH1 (mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli))
MLH1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MLH1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549 , H1299 , human MLH1-transfected 293T

Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.94 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: mutL homolog 1

Background: This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined.

Cellular Localization: Nucleus

Molecular Weight: 85 kDa

Gene ID: 4292

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response