HMGB1 antibody

Catalog No. ABIN2854997

The Rabbit Polyclonal anti-HMGB1 antibody has been validated for WB, IHC, IF, ICC, IHC (p), IHC (wm) and IHC (ff). It is suitable to detect HMGB1 in samples from Human. There are 4+ publications available.

Quick Overview for HMGB1 antibody (ABIN2854997)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm)), Immunohistochemistry (Free Floating) (IHC (ff))

Grade: KO Validated

Product Details anti-HMGB1 Antibody

Cross-Reactivity: Human, Mouse, Pig, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to HMGB1 (high-mobility group box 1)
HMGB1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12 , HMGB1-transfected 293T

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.46 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HMGB1 antibody (ABIN2854997)

Sixto-López, Rosales-Hernández, Contis-Montes de Oca, Fragoso-Morales, Mendieta-Wejebe, Correa-Basurto, Abarca-Rojano, Correa-Basurto: "N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells." in: International journal of molecular sciences, Vol. 21, Issue 16, (2020) (PubMed).

Contis-Montes de Oca, Rodarte-Valle, Rosales-Hernández, Abarca-Rojano, Rojas-Hernández, Fragoso-Vázquez, Mendieta-Wejebe, Correa-Basurto, Vázquez-Moctezuma, Correa-Basurto: "N-(2'-Hydroxyphenyl)-2-propylpentanamide (OH-VPA), a histone deacetylase inhibitor, induces the release of nuclear HMGB1 and modifies ROS levels in HeLa cells." in: Oncotarget, Vol. 9, Issue 70, pp. 33368-33381, (2018) (PubMed).

Bhatia, OBrien, Chen, Wong, Garner, Woodley, Li: "Dual therapeutic functions of F-5 fragment in burn wounds: preventing wound progression and promoting wound healing in pigs." in: Molecular therapy. Methods & clinical development, Vol. 3, pp. 16041, (2016) (PubMed).

Juranek, Daffu, Wojtkiewicz, Lacomis, Kofler, Schmidt: "Receptor for Advanced Glycation End Products and its Inflammatory Ligands are Upregulated in Amyotrophic Lateral Sclerosis." in: Frontiers in cellular neuroscience, Vol. 9, pp. 485, (2016) (PubMed).

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: high mobility group box 1

Background: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Cellular Localization: Nucleus

Molecular Weight: 25 kDa

Gene ID: 3146

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome