GAPDH antibody

Catalog No. ABIN285500

The Sheep Polyclonal anti-GAPDH antibody has been validated for WB and ELISA. It is suitable to detect GAPDH in samples from Human. There are 5+ publications available.

Quick Overview for GAPDH antibody (ABIN285500)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GAPDH Antibody

Purification: Purified

Immunogen: GAPDH antibody was raised in sheep using a 12 amino acid synthetic peptide (HQVVSSDFNSDT) representing the most conserved region of human and rat GAPDH conjugated with KLH as the immunogen.

Application Details

Application Notes: Optimal conditions to be determined by end user

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.2 with 0.1 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, NaN3 may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

References for anti-GAPDH antibody (ABIN285500)

Kuroda, Ishii, Uematsu, Ohata, Coban, Akira, Aritake, Urade, Morimoto: "Silica crystals and aluminum salts regulate the production of prostaglandin in macrophages via NALP3 inflammasome-independent mechanisms." in: Immunity, Vol. 34, Issue 4, pp. 514-26, (2011) (PubMed).

Blish, Clausen, Hawkins, Garvin, Willingham, Turner, Torti, Torti: "Loss of heterozygosity and SOSTDC1 in adult and pediatric renal tumors." in: Journal of experimental & clinical cancer research : CR, Vol. 29, pp. 147, (2011) (PubMed).

Heineke, Wollert, Osinska, Sargent, York, Robbins, Molkentin: "Calcineurin protects the heart in a murine model of dilated cardiomyopathy." in: Journal of molecular and cellular cardiology, Vol. 48, Issue 6, pp. 1080-7, (2010) (PubMed).

Shi, Zhang, Yang, Zhang, Wei: "ROCK1 plays an essential role in the transition from cardiac hypertrophy to failure in mice." in: Journal of molecular and cellular cardiology, Vol. 49, Issue 5, pp. 819-28, (2010) (PubMed).

Matsumoto, Takahashi, Shiva, Kawanishi, Kremenik, Kato, Yano: "The reduction of voluntary physical activity after poly I:C injection is independent of the effect of poly I:C-induced interferon-beta in mice." in: Physiology & behavior, Vol. 93, Issue 4-5, pp. 835-41, (2008) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH