BCL6 antibody (Internal Region)

Catalog No. ABIN2855012

The Rabbit Polyclonal anti-BCL6 antibody has been validated for WB, IHC (p), IP and ChIP. It is suitable to detect BCL6 in samples from Human and Mouse.

Quick Overview for BCL6 antibody (Internal Region) (ABIN2855012)

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Product Details anti-BCL6 Antibody

Binding Specificity: Internal Region

Characteristics: Rabbit Polyclonal antibody to BCL6 (B-cell CLL/lymphoma 6)
BCL6 antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human BCL6. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ChIP assay Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: Jurkat , Raji , NCI-H929 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: BCL6

Background: The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

Cellular Localization: Nucleus

Molecular Weight: 79 kDa

Gene ID: 604

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus