ALDOB antibody

Catalog No. ABIN2855024

The Rabbit Polyclonal anti-ALDOB antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect ALDOB in samples from Human. There are 2+ publications available.

Quick Overview for ALDOB antibody (ABIN2855024)

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDOB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-ALDOB Antibody

Cross-Reactivity: Human, Mouse, Rat, Turtle

Characteristics: Rabbit Polyclonal antibody to Aldolase B (aldolase B, fructose-bisphosphate)
Aldolase B antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro2A , GL261 , C8D30 , PC-12 , mouse brain , rat brain , 293T , A431 , HeLa , HepG2

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.09 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ALDOB antibody (ABIN2855024)

Chen, Lin, Chen, Cheng, Cheng, Imai et al.: "Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass ..." in: PLoS ONE, Vol. 15, Issue 1, pp. e0227838, (2020) (PubMed).

Dawson, Biggar, Storey: "Characterization of fructose-1,6-bisphosphate aldolase during anoxia in the tolerant turtle, Trachemys scripta elegans: an assessment of enzyme activity, expression and structure." in: PLoS ONE, Vol. 8, Issue 7, pp. e68830, (2014) (PubMed).

Target Details for ALDOB

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Alternative Name: aldolase, fructose-bisphosphate B

Background: Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5 % of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Molecular Weight: 39 kDa

Gene ID: 229

UniProt: P05062