Lactate Dehydrogenase A antibody

Catalog No. ABIN2855034

This anti-Lactate Dehydrogenase A antibody is a Rabbit Polyclonal antibody detecting Lactate Dehydrogenase A in WB, IF, ICC and IHC (p). Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for Lactate Dehydrogenase A antibody (ABIN2855034)

Target: Lactate Dehydrogenase A (LDHA)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lactate Dehydrogenase A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Lactate Dehydrogenase A Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Lactate Dehydrogenase (lactate dehydrogenase A)
LDHA antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human LDHA. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: PC-12 , Rat2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.33 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Lactate Dehydrogenase A antibody (ABIN2855034)

Thompson, Alvarez, Singha, Pavesic, Nguyen, Nelson, Fruman, Razorenova: "Targeting the Mevalonate Pathway Suppresses VHL-Deficient CC-RCC through an HIF-Dependent Mechanism." in: Molecular cancer therapeutics, Vol. 17, Issue 8, pp. 1781-1792, (2019) (PubMed).

Zera, Zastre: "Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes." in: PLoS ONE, Vol. 12, Issue 10, pp. e0186707, (2017) (PubMed).

Ugun-Klusek, Tatham, Elkharaz, Constantin-Teodosiu, Lawler, Mohamed, Paine, Anderson, John Mayer, Lowe, Ellen Billett, Bedford: "Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway." in: Cell death & disease, Vol. 8, Issue 1, pp. e2531, (2017) (PubMed).

Target Details for Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: lactate dehydrogenase A

Background: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Cellular Localization: Cytoplasm

Molecular Weight: 37 kDa

Gene ID: 3939

UniProt: P00338

Pathways: Warburg Effect