EIF3D antibody

Catalog No. ABIN2855038

This Rabbit Polyclonal antibody specifically detects EIF3D in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for EIF3D antibody (ABIN2855038)

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3D antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-EIF3D Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to EIF3D (eukaryotic translation initiation factor 3, subunit D)
EIF3D antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human EIF3D. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLaS3 , mouse liver , PC-12 , Rat2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.63 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EIF3D

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Alternative Name: eukaryotic translation initiation factor 3 subunit D

Background: Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex.

Cellular Localization: Cytoplasm

Molecular Weight: 64 kDa

Gene ID: 8664

UniProt: O15371

Pathways: Ribonucleoprotein Complex Subunit Organization