COX5A antibody (Center)

Catalog No. ABIN2855063

Product Details anti-COX5A Antibody

Target: COX5A (Cytochrome C Oxidase Subunit Va (COX5A))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX5A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Monkey, Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Monkey (97 %), Rat (93 %), Bovine (93 %)

Characteristics: Rabbit Polyclonal antibody to COX5A (cytochrome c oxidase subunit Va)
COX5A antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human COX5A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for COX5A

Target: COX5A (Cytochrome C Oxidase Subunit Va (COX5A))

Alternative Name: COX5A (COX5A Products)

Synonyms: COX antibody, COX-VA antibody, VA antibody, CG14724 antibody, COXA_DROME antibody, Dmel\\CG14724 antibody, Va antibody, tend antibody, cox5a antibody, cb01f09 antibody, mg:cb01f09 antibody, GB15816 antibody, MGC79007 antibody, Afu5g02750 antibody, wu:fj32b09 antibody, zgc:109989 antibody, zgc:114165 antibody, AA959768 antibody, CcOX antibody, cytochrome c oxidase subunit 5A antibody, Cytochrome c oxidase subunit 5A antibody, cytochrome c oxidase subunit Vab antibody, cytochrome c oxidase subunit 5A, mitochondrial antibody, cytochrome c oxidase subunit Va S homeolog antibody, cytochrome c oxidase subunit Va antibody, cytochrome C oxidase subunit Va antibody, cytochrome c oxidase subunit Vaa antibody, COX5A antibody, cox5ab antibody, LOC408837 antibody, cox5a.S antibody, cox5a antibody, Cox5a antibody, CoVa antibody, AFUA_5G02750 antibody, IscW_ISCW008682 antibody, LOC100134927 antibody, cox5aa antibody, LOC100988094 antibody

Background: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.

Cellular Localization: Mitochondrion inner membrane

Molecular Weight: 17 kDa

Gene ID: 9377

Pathways: Proton Transport