LGALS1/Galectin 1 antibody

Catalog No. ABIN2855093

The Rabbit Polyclonal anti-LGALS1/Galectin 1 antibody has been validated for WB, ICC, IP, IF, IHC (p), IHC (fro) and IHC (wm). It is suitable to detect LGALS1/Galectin 1 in samples from Human. There are 2+ publications available.

Quick Overview for LGALS1/Galectin 1 antibody (ABIN2855093)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGALS1/Galectin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Whole Mount) (IHC (wm))

Grade: KO Validated

Product Details anti-LGALS1/Galectin 1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Galectin 1 (lectin, galactoside-binding, soluble, 1)
Galectin 1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Galectin 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NCI-H929 , BCL-1 , PC-12 , Rat2 , HeLa

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.52 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-LGALS1/Galectin 1 antibody (ABIN2855093)

Borowski, Tirado-Gonzalez, Freitag, Garcia, Barrientos, Blois: "Altered Glycosylation Contributes to Placental Dysfunction Upon Early Disruption of the NK Cell-DC Dynamics." in: Frontiers in immunology, Vol. 11, pp. 1316, (2020) (PubMed).

Paz, Joo, Chou, Fei, Mayo, Abdel-Azim, Ghazarian, Groffen, Heisterkamp: "Treatment of B-cell precursor acute lymphoblastic leukemia with the Galectin-1 inhibitor PTX008." in: Journal of experimental & clinical cancer research : CR, Vol. 37, Issue 1, pp. 67, (2019) (PubMed).

Target Details for LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: galectin 1

Background: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.

Cellular Localization: Secreted , extracellular space , extracellular matrix

Molecular Weight: 15 kDa

Gene ID: 3956

UniProt: P09382

Pathways: Carbohydrate Homeostasis