DOK1 antibody

Catalog No. ABIN2855104

This Rabbit Polyclonal antibody specifically detects DOK1 in WB and IHC (p). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for DOK1 antibody (ABIN2855104)

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DOK1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to DOK1 (docking protein 1, 62 kDa (downstream of tyrosine kinase 1))
DOK1 antibody [N1C1]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human p62Dok. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Raji

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-DOK1 antibody (ABIN2855104)

Sarin, Engel, Rothweiler, Cinatl, Michaelis, Frötschl, Fröhlich, Kalayda: "Key Players of Cisplatin Resistance: Towards a Systems Pharmacology Approach." in: International journal of molecular sciences, Vol. 19, Issue 3, (2018) (PubMed).

Target Details for DOK1

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Alternative Name: docking protein 1

Background: Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Molecular Weight: 52 kDa

Gene ID: 1796

UniProt: Q99704