CHD4 antibody (N-Term)

Catalog No. ABIN2855116

This Rabbit Polyclonal antibody specifically detects CHD4 in WB, IF, IP, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CHD4 antibody (N-Term) (ABIN2855116)

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHD4 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to CHD4 (chromodomain helicase DNA binding protein 4)
CHD4 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human CHD4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , Raji , K562

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.48 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CHD4

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Alternative Name: chromodomain helicase DNA binding protein 4

Background: The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.

Cellular Localization: Nucleus

Molecular Weight: 218 kDa

Gene ID: 1108

UniProt: Q14839