AP2B1 antibody (Internal Region)

Catalog No. ABIN2855154

Product Details anti-AP2B1 Antibody

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP2B1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Bovine (100 %)

Characteristics: Rabbit polyclonal antibody to beta-Adaptin (adaptor-related protein complex 1, beta 1 subunit)
beta-Adaptin antibody [N3C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human beta-Adaptin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for AP2B1

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Alternative Name: beta-Adaptin (AP2B1 Products)

Background: Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Two transcript variants encoding different isoforms have been found for this gene, and variants utilizing alternative polyadenylation signals exist.

Cellular Localization: Golgi apparatus , Cytoplasmic vesicle , clathrin-coated vesicle membrane

Molecular Weight: 105 kDa

Gene ID: 162

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation