Actin, gamma 1 antibody (N-Term)

Catalog No. ABIN2855174

The Rabbit Polyclonal anti-Actin, gamma 1 antibody has been validated for WB, IHC (p) and IHC (fro). It is suitable to detect Actin, gamma 1 in samples from Human. There are 3+ publications available.

Quick Overview for Actin, gamma 1 antibody (N-Term) (ABIN2855174)

Target: Actin, gamma 1 (ACTG1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin, gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Actin, gamma 1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Cat, Dog, Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to alpha Sarcomeric Actin (actin, cytoplasmic 2)
alpha Sarcomeric Actin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Actin Gamma 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , JC , BCL-1 , A431 , PC-12 , Rat2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 1XPBS ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Actin, gamma 1 antibody (ABIN2855174)

Xie, Perrino: "Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions." in: Analytical biochemistry, Vol. 577, pp. 1-13, (2020) (PubMed).

Chen, Wang, Wei, Tzeng, Lan, Cheng, Kuo: "Maintenance BEZ235 Treatment Prolongs the Therapeutic Effect of the Combination of BEZ235 and Radiotherapy for Colorectal Cancer." in: Cancers, Vol. 11, Issue 8, (2019) (PubMed).

Li, Sasse, Bayguinov, Ward, Perrino: "Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes." in: Journal of diabetes research, Vol. 2018, pp. 8743874, (2018) (PubMed).

Target Details for Actin, gamma 1

Target: Actin, gamma 1 (ACTG1)

Alternative Name: actin gamma 1

Background: Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Cellular Localization: Cytoplasm , cytoskeleton

Molecular Weight: 42 kDa

Gene ID: 71

UniProt: P63261

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization