F4/80 antibody (C-Term)

Catalog No. ABIN2855220

Cited in 1 publication. The Rabbit Polyclonal anti-F4/80 antibody (ABIN2855220) specifically detects F4/80 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for F4/80 antibody (C-Term) (ABIN2855220)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This F4/80 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-F4/80 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to EMR1 (egf-like module containing, mucin-like, hormone receptor-like 1)
EMR1 antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human F4/80. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: rat liver , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.23 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-F4/80 antibody (ABIN2855220)

Dekker, van Geemen, van den Bogaerdt, Driessen-Mol, Aikawa, Smits: "Sheep-Specific Immunohistochemical Panel for the Evaluation of Regenerative and Inflammatory Processes in Tissue-Engineered Heart Valves." in: Frontiers in cardiovascular medicine, Vol. 5, pp. 105, (2018) (PubMed).

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: adhesion G protein-coupled receptor E1

Background: This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties.

Cellular Localization: Cell membrane, Multi-pass membrane protein

Molecular Weight: 98 kDa

Gene ID: 2015

UniProt: Q14246