EIF3I antibody

Catalog No. ABIN2855240

The Rabbit Polyclonal anti-EIF3I antibody has been validated for WB, IF and ICC. It is suitable to detect EIF3I in samples from Human.

Quick Overview for EIF3I antibody (ABIN2855240)

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3I antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-EIF3I Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to EIF3 beta (eukaryotic translation initiation factor 3, subunit I)
EIF3 beta antibody [N2C3]

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human EIF3 beta. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 mg/mL

Buffer: 1XPBS ( pH 7), 40 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EIF3I

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Alternative Name: eukaryotic translation initiation factor 3 subunit I

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Molecular Weight: 37 kDa

Gene ID: 8668

UniProt: Q13347

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Ribonucleoprotein Complex Subunit Organization, Synthesis of DNA