EIF3F antibody

Catalog No. ABIN2855242

Product Details anti-EIF3F Antibody

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to EIF3F (eukaryotic translation initiation factor 3, subunit F)
EIF3F antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human EIF3F. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.39 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EIF3F

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Alternative Name: eukaryotic translation initiation factor 3 subunit F (EIF3F Products)

Synonyms: AO090005001590 antibody, Dmel\CG9769 antibody, eIF3-S5 antibody, eif3f antibody, LOC733116 antibody, eIF3f antibody, EIF3S5 antibody, eIF3-p47 antibody, 0610037M02Rik antibody, AA409853 antibody, Eif3s5 antibody, eukaryotic translation initiation factor 3 subunit F antibody, hypothetical protein antibody, CG9769 gene product from transcript CG9769-RA antibody, eukaryotic translation initiation factor 3 subunit 5 antibody, eukaryotic translation initiation factor 3, subunit F antibody, Eukaryotic translation initiation factor 3 subunit F antibody, eif3f antibody, ANI_1_120074 antibody, AOR_1_2780174 antibody, PTRG_03897 antibody, PAAG_00376 antibody, MCYG_08502 antibody, MGYG_08653 antibody, PGTG_04587 antibody, CG9769 antibody, LOC733116 antibody, EIF3F antibody, Eif3f antibody, eif-3.F antibody

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Cellular Localization: Cytoplasm

Molecular Weight: 38 kDa

Gene ID: 8665

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization