ARPC2 antibody

Catalog No. ABIN2855249

The Rabbit Polyclonal anti-ARPC2 antibody has been validated for WB, IF, ICC and IHC (p) and has been independently validated. It is suitable to detect ARPC2 in samples from Human.

Quick Overview for ARPC2 antibody (ABIN2855249)

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARPC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARPC2 Antibody

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to ARPC2 (actin related protein 2/3 complex, subunit 2, 34 kDa)
ARPC2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ARPC2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: rat kidney , mouse kidney

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104342 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104342

Date: 04/20/2021

Antigen: ARPC2

Lot Number: 39911

Method validated: Multiplex Immunohistochemistry

Positive Control:

FFPE normal human cortex

Negative Control:

Unlabeled control (FFPE normal human cortex)

Notes:

Passed. The anti-ARPC2 antibody ABIN2855249 produced staining in neuronal processes throughout the human cortex.

Validation Images

FFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. MAP2, neurofilament, and GFAP were labeled with anti-MAP2 antibody ABIN125739 (green; conjugated to fluorophore ATTO 550), anti-neurofilament antibody (red; conjugated to fluorophore AF488), and anti-GFAP antibody (purple; conjugated to fluorophore AF647) respectively.

FFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. GFAP was labeled with anti-ARPC2 antibody (red; conjugated to fluorophore AF647).

Full Methods

Primary Antibody: ABIN2855249

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-ARPC2 antibody ABIN2855249 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.

Experimental Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of ARPC2 was observed with human FFPE cortical tissue sections with tris-EDTA antigen retrieval.

• Anti-ARPC antibody ABIN2855249 was weakly compatible with staining of fresh frozen mouse tissue

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ARPC2

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Alternative Name: actin related protein 2/3 complex subunit 2

Background: This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Cellular Localization: Cytoplasm , cytoskeleton , Cell projection

Molecular Weight: 34 kDa

Gene ID: 10109

UniProt: O15144

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization