KARS antibody (N-Term)

Catalog No. ABIN2855312

The Rabbit Polyclonal anti-KARS antibody has been validated for WB and IHC (p). It is suitable to detect KARS in samples from Human.

Quick Overview for KARS antibody (N-Term) (ABIN2855312)

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KARS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KARS Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to Lysyl-tRNA synthetase (lysyl-tRNA synthetase)
Lysyl-tRNA synthetase antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Lysyl-tRNA synthetase. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.77 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for KARS

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Alternative Name: lysyl-tRNA synthetase

Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm

Molecular Weight: 68 kDa

Gene ID: 3735

UniProt: Q15046